24 Characters Total. 14 Starters + 10 Unlockables. All unlocked via Grand Prix (GP) cups or Time Trials.
| Character | Method | Notes |
|---|---|---|
| Baby Daisy | Win 100cc Star Cup | Or 1,200 races |
| Baby Luigi | Win 100cc Leaf Cup | Or 800 races |
| Baby Peach | Win 100cc Flower Cup | Or 400 races |
| Daisy | Win 50cc Star Cup | Or 3,200 races |
| Diddy Kong | Win 50cc Leaf Cup | Or 2,400 races |
| King Boo | Win 50cc Flower Cup | Or 1,600 races |
| Rosalina | Win 150cc Mirror Star Cup | Or 4,800 races / Super Mario Galaxy save |
| Funky Kong | Win 150cc Mirror Leaf Cup | Or 3,600 races / 20 Ghost Data |
| Dry Bones | Win 150cc Mirror Flower Cup | Or 2,800 races |
| Toadette | Win 150cc Mirror Mushroom Cup | Or 2,000 races |
| Waluigi | Win 150cc Mirror Shell Cup | Or 3,200 races |
| Dry Bowser | Gold Trophy on ALL 32 Time Trial Tracks | Hardest unlock. Requires mastering every track. |
Karts/Bikes unlocked via Time Trials (staff ghosts) and Grand Prix (cups). Check a vehicle unlock chart.