| Character | Requirement |
|---|---|
| Daisy | Win 50cc World Championship |
| Waluigi | Win 100cc World Championship |
| Rosalina | Win 150cc World Championship |
| Dry Bones | Win Mirror World Championship |
| King Boo | Win 50cc with Staff Ghost |
| Toadette | Win 100cc with Staff Ghost |
| Baby Mario | Win 150cc with Staff Ghost |
| Baby Luigi | Win Mirror with Staff Ghost |
| Baby Peach | Win 50cc with Staff Ghost |
| Baby Daisy | Win 100cc with Staff Ghost |
| Baby Bowser | Win 150cc with Staff Ghost |
| Metal Mario | Win Mirror with Staff Ghost |
Extra karts, bikes, and wheels unlock via separate challenges.