No. Pit has no unique voice line for picking up items (including Poké Balls, Assist Trophies, or standard items) in any Smash game (Brawl, 3DS/Wii U, Ultimate).
| Trigger | Line |
|---|---|
| Match Start | "I'm going to win!" / "Time to shine!" |
| KO / Star KO | "Noooo!" / "Icarus...!" |
| Victory Screen | "Victory is mine!" / "Lady Palutena, I did it!" |
| Taunt (Up) | "You're not ready for me!" |
| Taunt (Side) | "Hyah! Hyah! Hyah!" |
| Taunt (Down) | Prays / "Palutena, guide me." |
| Final Smash (Three Sacred Treasures) | "Three Sacred Treasures! Grant me power!" |
He plays generic grunts / "Hyah!" on throw/attack with item—same as most characters. No item-specific dialogue.
Source: Smash Wiki voice clip rips + in-game testing.