No. The DSi removed the GBA cartridge slot (Slot‑2) that was present on the original DS and DS Lite.
| Method | How | Pros | Cons |
|---|---|---|---|
| Flashcart (Kernel) | R4i Gold 3DS Plus, Ace3DS X + GBARunner2 homebrew | Plays GBA ROMs from SD | ~85% compatibility; no link cable; sleep‑mode issues |
| Slot‑2 Flashcart (DS/DS Lite only) | EZ‑Flash Omega, EverDrive GBA X5 | 100% hardware accuracy | Won’t fit DSi/3DS |
| Emulation (PC/Phone/Steam Deck) | mGBA, RetroArch | Perfect, shaders, save states, netplay | Not on DSi hardware |
| Wii U Virtual Console | Select GBA titles (eShop closed 2023) | Official, legal | Limited library; Wii U only |
Best experience: Play GBA on original hardware (AGS‑101 + EZ‑Flash Omega) or a modern emulator.