Assassin's Creed 2: Single-Player Only, No Multiplayer

No. Assassin's Creed 2 is not a two-player game. It is a single-player action-adventure game where you control Ezio Auditore da Firenze.

While later Assassin's Creed titles introduced multiplayer modes, the core experience of Assassin's Creed 2 is entirely single-player.